FluorCamDesktop Plant Multispectral Fluorescent Imaging System

<unk> Teknoloji instrumental ye luɔɔi apɛi në puɔ̈ɔ̈c de phytotypes ku physiological ecological experiments

PSIProfesɛr Nedbal, raan ŋic käŋ ŋic käŋ ŋic käŋ ŋic käŋ ŋic käŋ ŋic käŋ ŋic käŋ ŋic käŋ ŋic käŋ ŋic käŋ ŋic käŋ ŋic käŋ ŋic käŋ ŋic käŋ ŋic käŋ ŋic käŋ ŋic käŋ ŋic käŋ ŋic käŋ ŋic käŋ ŋic käŋ ŋic käŋ ŋic Nedbal et al., 2000; Govindjee and Nedbal, 2000）。 FluorCam fluorescence imaging technology ye kɛ̈ɛ̈r ye kɛ̈ɛ̈r ye kɛ̈ɛ̈r ye kɛ̈ɛ̈r ye kɛ̈ɛ̈r ye kɛ̈ɛ̈r ye kɛ̈ɛ̈r ye kɛ̈ɛ̈r ye kɛ̈ɛ̈r ye kɛ Në ye mɛn, PSI Corporation acï bɛ̈n ya raan cï puɔ̈l apɛi, raan cï puɔ̈l apɛi, raan cï puɔ̈l apɛi, raan cï puɔ̈l apɛi, raan cï puɔ̈l apɛi

Fotosynthesis Research, 66: 3-12, 2000) ye FluorCam fluorescence imaging technology ye Nedbal ku kɔc kɔ̈k looi në run 1990 yiic. Fotosynthesis Research, 38: 571-579, 2000)

FluorCamDesktop plant multi-spectrum fluorescence imaging system is a highly integrated, highly innovative, easy to use, widely used high-end plant live imaging technology equipment, high sensitivity CCD lenses, 4 fixed LED light source boards and control systems etc. integrated into a dark adaptation operating box (can also be selected according to requirements with a fifth light source board placed at the top), plant samples placed on the partition board within the dark adaptation operating box, the partition board level 7 height adjustable. Light source provides power from high stability power supply unit, 4 high energy, high stability LED light source board all uniformly lit on plant samples, imaging area can reach 13. Light source provides power from high stability power supply unit, 4 high energy, high stability LED light source board all uniformly lit on plant samples, imaging area can reach 13.×13 cmKɔmpiöta ye kɔmpiöta ye kɔmpiöta ye kɔmpiöta ye kɔmpiöta ye kɔmpiöta ye kɔmpiöta ye kɔmpiöta ye kɔmpiöta ye kɔmpiöta ye kɔmpiöta ye kɔmpiöta ye kɔmpiöta A tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛ

Kä ye looi:

· Sisitɛm ye tɔ̈ɔ̈u në dark adaptation operating box, ye luɔɔi thiin koor, ye rot waar, a lëu bï dark adaptation imaging measurement analysis looi në laboratory yic wala yɔ̈ɔ̈r

· CCD lens ye cɔl high sensitivity, time resolution up to 50 photos per second, quick capture of chlorophyll fluorescence transients, imaging area up to 13x13cm

· A ye teknoloji ye cɔl fluorescence fluorescence ye cɔl fluorescence fluorescence ye cɔl fluorescence fluorescence ye cɔl fluorescence ye cɔl fluorescence ye cɔl fluorescence ye cɔl fluorescence ye cɔl fluorescence ye cɔl fluorescence ye cɔl fluorescence ye cɔl fluorescence ye cɔl fluorescence ye cɔl fluorescence ye cɔl fluorescence ye cɔl fluorescence ye cɔl fluorescence ye cɔl fluorescence ye cɔl fluorescence ye cɔl fluorescence ye cɔl fluorescence ye cɔl fluorescence ye cɔl

· A ye duniɛ dɛɛ̈ɛ̈r ye kɛ̈n ye kɛ̈n ye kɛ̈n ye kɛ̈n ye kɛ̈n ye kɛ̈n ye kɛ̈n ye kɛ̈n ye kɛ̈n ye kɛ̈n ye kɛ̈n ye kɛ̈n ye kɛ̈n100µsA lëu bï ya 120,000 μmol ((photons) / m².s

· Protocols (Protocols) ye kɛ̈ɛ̈l ye kɛ̈ɛ̈l ye kɛ̈ɛ̈l ye kɛ̈ɛ̈l ye kɛ̈ɛ̈l ye kɛ̈ɛ̈l ye kɛ̈ɛ̈l ye kɛ̈ɛ̈l ye kɛ̈ɛ̈l ye kɛ̈ɛ̈l ye kɛ̈ɛ̈l ye kɛ̈ɛ̈l ye kɛ̈ɛ̈l ye kɛ̈ɛ̈l ye kɛ̈ɛ̈l ye kɛ̈ɛ̈l ye kɛ̈ɛ̈l

· A lëu bï ya looi automatique repeat imaging measurement analysis, ka tɛ̈n yenë a tɛ̈n yenë a tɛ̈n yenë a tɛ̈n yenë a tɛ̈n yenë a tɛ̈n yenë a tɛ̈n yenë a tɛ̈n yenë a tɛ̈n yenë a tɛ̈n yenë a tɛ̈n yenë a tɛ A lëu bï ya tɛ̈n ya tɛ̈n ya tɛ̈n ya tɛ̈n ya tɛ̈n ya tɛ̈n ya tɛ̈n ya tɛ̈n ya tɛ̈ Cïtmɛn, ka Fv/Fm tɛ̈n tɛ̈n tɛ̈n tɛ̈n tɛ̈n tɛ̈n tɛ̈n tɛ̈n tɛ̈n tɛ̈n tɛ̈n

· A tɔŋ fotokemikal ye kɔnɔŋ ye kɔnɔŋ ye kɔnɔŋ ye kɔnɔŋ ye kɔnɔŋ ye kɔnɔŋ ye kɔnɔŋ ye kɔnɔŋ ye kɔnɔŋ ye kɔnɔŋ ye kɔnɔŋ ye kɔnɔŋ ye kɔnɔŋ ye kɔnɔŋ ye kɔnɔŋ ye kɔnɔŋ ye kɔnɔŋ

A ye Fv/Fm ye kɛ̈ɛ̈r ye kɛ̈ɛ̈r ye kɛ̈ɛ̈r ye kɛ̈ɛ̈r ye kɛ̈ɛ̈r ye kɛ̈ɛ̈r ye kɛ̈ɛ̈r ye kɛ̈ɛ̈r Kɔrɔ ye fotosynthesis ye kɔrɔ ye kɔrɔ ye kɔrɔ ye kɔrɔ ye kɔrɔ ye kɔrɔ ye kɔrɔ ye kɔrɔ ye kɔrɔ ye kɔrɔ ye kɔrɔ ye kɔrɔ ye kɔrɔ ye kɔrɔ ye kɔrɔ ye kɔrɔ ye kɔrɔ ye kɔrɔ ye kɔrɔ

· Chlorophyl fluorescence imaging, multi-spectral fluorescence imaging, GFP steady state fluorescence imaging

· TetraCam color imaging module, maximum imaging area 20x25cm, for leaf or plant morphology imaging analysis and chlorophyll fluorescence imaging contrast analysis

· A lëu bï ya tɔ̈ɔ̈u në spectral imaging unit ku infrared thermal imaging unit, plant characteristics digitalization, visualization, comprehensive measurement and analysis of plant morphology, photosynthesis efficiency, biochemical properties, air pore conductivity, forcing and resistance etc.

· A lëu bï ya tɔ̈ɔ̈u ka tɔ̈ɔ̈u ka tɔ̈ɔ̈u ka tɔ̈ɔ̈u ka tɔ̈ɔ̈u ka tɔ̈ɔ̈u ka tɔ̈ɔ̈u ka tɔ̈ɔ̈u ka tɔ̈ɔ̈u ka tɔ̈ɔ̈u

Kä ye looi:

Hendrik Kupper"Plant Physiology" cï gɔ̈t në pɛɛi de thiɛ̈ɛ̈r 2019, ku Zuzana Benedikty et al. Analysis of OJIP Chlorophyll Fluorescence Kinetics and QA Reoxidation Kinetics by Direct Fast Imaging， Ka tɛmɛ, ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka tɛmɛ ka 4000fps@640x512 ， QA re-oxidation chlorophyll fluorescence dynamic imaging measure single pulse saturated light flash150,000μmol/m2s.1Ye.

A: OJIP quick fluorescence dynamics determination analysis parameters include:

a)Fo: Fluorescence ye daminɛ wala fluorescence ye cɔl minimum, fluorescence ye 50 μs

b)Fj ye: Fluorescence në 2ms

c)FiFluorescence në 60ms

d)PWala Fm: maximum fluorescence

e)Vj= ((Fj-Fo) / ((Fm-Fo): Fluorescence Relative Variable

f)Vi ye= (Fi-Fo) / (Fm-Fo): I-class fluorescence relative variable

g)Mo= TRo/RC-ETo/RC=4(F300-Fo)/(Fm-Fo): Fluorescent transient initial slope, wala OJIP curve initial slope

h)Daan: OJIP curve and Fm area, can be called complementary area. To compare different samples, Area needs to be standardized as: Sm = Area/(Fm-Fo), Sm is a measure of the required energy to shut down all light response centers.

i)Fix Area: OJIP fixed area, OJIP curve 40 subtle when F value to 1 second when F value below the area: OJIP fixed area, OJIP curve 40 subtle when F value to 1 second when F value below the area

j)Sm: OJIP compensation area standardized, ye nyuɔɔth QA reduction juëc turnover

k)Ss= Vj/Mo: OJ phase compensation area, ye nyuɔɔth single-cycle QA reduction

l)N=Sm/Ss=Sm Mo ((1/Vj)OJIP QA (kam 0 ku t)_Fm）

m)Phi-Po＝QY＝φpo＝TRo/ABS＝Fv/Fm， Maximum light quantum output, absorbing light quantum flow response center initial capture ratio

n)Psi_o＝ψo＝ETo/TRo＝1-Vj， Quantum flow ratio of electron transmitting light in capturing light quantum flow

o)Phi_Eo＝φ_Eo=ETo/ABS=(1-(Fo/Fm))(1-Vj)， Quantum yield of electron transport at t=0 (Quantum yield of electron transport at t=0) (Quantum yield of electron transport at t=0) (Quantum yield of electron transport at t=0) (Quantum yield of electron transport at t=0)

p)Phi_Do＝φ_Do＝1-φpo＝Fo/Fm， Kä ye kek looi në quantum yic (t=0)

q)Phi_pav= φpav = φpo ((Sm/t)_Fm), average light quantum yield, t_FmWakt tɔ̈u bï Fm ya tɔ̈u (ms)

r)ABS/RC= Mo ((1/Vj) ((1/QY): ye kɔc ye kɔc ye kɔc ye kɔc ye kɔc ye kɔc ye kɔc ye kɔc ye kɔc ye kɔc ye kɔc ye kɔc ye kɔc ye kɔc ye kɔc ye kɔc ye kɔc ye kɔc ye kɔc ye kɔc ye kɔc ye kɔc ye kɔc ye kɔc ye kɔckä ye kek looi (QA to QA<unk> reducing)(Dɔɔr). QY=TRo/ABS=Fv/Fm

s)TRo/RC= Mo ((1/Vj): unit reaction center initial (or maximum) capture light quantum flow (causing a reduction of QA, i.e. increasing the ratio of closure of the reaction center B): unit reaction center initial (or maximum) capture light quantum flow (causing a reduction of QA, i.e. increasing the ratio of closure of the reaction center B): unit reaction center initial (or maximum) capture light quantum flow

t)ETo/RC=Mo(1/Vj) (1-Vj): Kä ye kek looi në ɣän ke kɔc ye röt looi në ɣän ke kɔc ye röt looi në ɣän ke kɔc ye röt looi në ɣän ke kɔc ye röt looi në ɣän ke kɔc ye röt looi

u)DIo/RC= (ABS/RC) - (TRo/RC): Ajuiɛɛr de wɛ̈ɛ̈r de wɛ̈ɛ̈r de wɛ̈ɛ̈r de wɛ̈ɛ̈r de wɛ̈ɛ̈r

v)ABS/CS: Quantum flow of absorption light of unit sample section,CS yaa wal ye cɔl excited cross-section of the tested sample(Dɔɔr). ABS/CSo=Fo, ABS/CSm=Fm, TRo/CSx=QY ((ABS/CSx) <unk> unit section captures energy or light quantum flow

w)TRo/CSo= QY. Fo; ETo/CSo=φ_EoFo = QY. (1-Vj). Fo

x)RC/CSx: Reaction Center Density,RC / CS0 (active RCs per excited cross-section)

y)PI_ABS= ((RC/ABS) ((φpo/φ)_Do((ψo/Vj): "Performance" index wala survival index, ye rot looi në kë de kä ye kek looi në kë de kä ye kek looi në kë de kä ye kek looi në kë de kä ye kek looi në kë de kä ye kek looi në kë de kä ye kek looi

z)PIcs= ((RC/CSx) ((φpo/φ)_Do((ψo/Vj): "Performance" index wala survival index